2020
139.
Hammerling MJ, Yoesep DJ, Jewett MC (2020) “Single enzyme RT-PCR of full length ribosomal RNA” Synthetic Biology OUP5(1): ysaa028
138.
Gupta M, Wagner D, Jewett MC (2020) “Engineered proteins as multifunctional materials” MRS Bulletin 45(12): 999-1004
137.
Danielson N, McKay S, Bloom P, Dunn J, Jakel N, Bauer T, Hannon J, Jewett MC, Shanks B (2020) “Industrial Biotechnology—An Industry at an Inflection Point” Industrial Biotechnology 16(6): 321-332
136.
Hershewe JM, Wiseman WD, Kath JE, Buck CC, Gupta MK, Dennis PB, Naik RR, Jewett MC (2020) “Characterizing and controlling nanoscale self-assembly of suckerin-12” ACS Synthetic Biology 9(12): 3388-3399
135.
Hershewe JM, Kightlinger W, Jewett MC (2020) “Cell-free systems for accelerating glycoprotein expression and biomanufacturing” Journal of Industrial Microbiology and Biotechnology 47(11): 977-991
134.
Bogart JW, Cabezas MD, Vögeli B, Wong D, Karim AS, Jewett MC (2021) “Cell-free exploration of the natural product chemical space” ChemBioChem 22(1): 84-91
133.
Karim AS, Liew FE, Garg S, Vögeli B, Rasor BJ, Gonnot A, Pavan M, Juminaga A, Simpson SD, Köpke M, Jewett, MC (2020) “Modular cell-free expression plasmids to accelerate biological design in cells” Synthetic Biology OUP 5(1): ysaa019
132.
Zhu B, Gan R, Cabezas MD, Kojima T, Nicol R, Jewett MC, Nakano H (2020) “Increasing cell-free gene expression yields from linear templates in Escherichia coli and Vibrio natriegens extracts by using DNA-binding proteins” Bioengineering and Biotechnology 117(12): 3849-3857
131.
Liu WQ, Wu C, Jewett MC, Li J (2020) “Cell-free protein synthesis enables one-pot cascade biotransformation in an aqueous-organic biphasic system” Biotechnology and Bioengineering 117(12): 4001-4008
130.
Lee J, Schwarz KJ, Kim DS, Moore JS, Jewett MC (2020) “Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro” Nature Communications 11: 4304
129.
Thames AH, Wolniak KL, Stupp SI, Jewett MC (2020) “Principles learned from the international race to develop a safe and effective COVID-19 vaccine” ACS Central Science 6 (8): 1341–1347
128.
Jaroentomeechai T, Taw MN, Li M, Aquino A, Agashe N, Chung S, Jewett MC, DeLisa MP (2020) “Cell-free synthetic glycobiology: Designing and engineering glycomolecules outside of living cells” Frontiers in Chemistry 8: 645
127.
Grubbe WS, Rasor BJ, Krüger A, Jewett MC, Karim AS (2020) “Cell-free styrene biosynthesis at high titers” Metabolic Engineering 61: 89-95
126.
Dudley QM, Karim AS, Nash CJ, Jewett MC (2020) “In vitro prototyping of limonene biosynthesis using cell-free protein synthesis” Metabolic Engineering 61: 251-260
125.
Kightlinger W, Warfel K, DeLisa MP, Jewett MC (2020) “Synthetic Glycobiology: Parts, Systems, and Applications.” ACS Synthetic Biology 9(7): 1534–1562
124.
Krüger A, Mueller AP, Rybnicky GA, Engle NL, Yang ZK, Tschaplinski TJ, Simpson SD, Köpke M, Jewett MC (2020) “Development of a clostridia-based cell free system for prototyping genetic parts and metabolic pathways” Metabolic Engineering 62: 95-105
123.
Karim AS, Dudley QM, Juminga A, Yuan Y, Crowe SA, Heggestad JT, Garg S, Abdalla T, Grubbe W, Rasor B, Coar D, Torculas M, Krein M, Liew F, Quattlebaum A, Jensen RO, Stuart J, Simpson SD, Köpke M, Jewett MC (2020) “ In vitro prototyping and rapid optimization of biosynthetic enzymes for cellular design.” Nature Chemical Biology 16: 912–919
122.
Lee J, Torres R, Kim DS, Byrom M, Ellington AD, Jewett MC (2020) “Ribosomal incorporation of cyclic β-amino acids into peptides using in vitro translation” Chemical Communications 56: 5597-5600
121.
Whitehead TA, Banta S, Bentley WE, Betenbaugh MJ, Chane C, Clark DS, Hoeslig C, Jewett MC, Junkeri B, Koffas M, Kshirsagark R, Lewis A, Lid CT, Maranas C, Papoutsakis E, Prather KJ, Schaffer S, Segatori L, Wheeldon I (2020) “The importance and future of biochemical engineering” Biotechnology and Bioengineering 117(8): 2305-2318
120.
Aleksashin NA, Szal T, d’Aquino AE, Jewett MC, Mankin AM (2020) “A fully orthogonal system for protein synthesis in bacterial cells” Nature Communications 11: 1858
119.
Zhuang L, Huang S, Liu WQ, Karim AS, Jewett MC, Li, J. (2020) “Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin” Metabolic Engineering 60: 37-44
118.
Kennedy NW, Hershewe JM, Nichols TM, Roth EW, Wilke CD, Jewett MC, Tullman-Ercek D (2020) “Apparent size and morphology of bacterial microcompartments varies with technique” PLOS ONE 5(3): e0226395
117.
Jewett MC, Hammerling MJ (2020) “Ribosomes read new languages” Nature Chemical Biology 16: 486-488
116.
Chen Z, Kibler RD, Hunt A, Busch F, Pearl J, Jia M, VanAernum ZL, Wicky BIM, Dods G, Liao H, El-Samad H, Stamatoyannopoulos J, Wysocki VH, Jewett MC, Boyken SE, Baker D (2020) “De novo design of protein logic gates” Science 368: 78-84
115.
Thavarajah W, Verosloff MS, Jung JK, Alam KK, Miller JD, Jewett MC, Young SL, Lucks JB (2020) “A primer on emerging field-deployable synthetic biology tools for democratizing global water quality monitoring” NPJ Clean Water 3:18
114.
Hammerling MJ, Fritz BR, Yoesep DJ, Kim DS, Carlson ED, Jewett MC (2020) “In vitro ribosome synthesis and evolution through ribosome display” Nature Communications 11: 1108
113.
Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB (2020) “Design and optimization of a cell-free atrazine biosensor” ACS Synthetic Biology 9(3): 671-677
112.
Lin L, Kightlinger W, Hockenberry AJ, Jewett MC, Mrksich M (2020) “Sequential glycosylation of protein via substrate specific N-glycosyltransferases” ACS Central Science 6(2): 144-154
111.
d’Aquino AE, Azim T, Aleksashin NA, Hockenberry AJ, Jewett MC (2020) “Mutational characterization and mapping of the 70S ribosome active site” Nucleic Acids Research 48(5): 2777–2789
110.
Liu X, Silverman AD, Alam KK, Iverson E, Lucks JB, Jewett MC, Raman S (2020) “Design of a transcriptional biosensor for the portable, on-demand detection of cyanuric acid” ACS Synthetic Biology 9(1): 84-94
109.
Silverman AD, Karim AS, Jewett MC (2020) “Cell-free gene expression systems: An expanding repertoire of applications” Nature Reviews Genetics 21: 151-170
108.
Thavarajah W, Silverman AS, Verosloff MS, Kelley-Loughnane N, Jewett MC, Lucks JB (2020) “Point-of-use detection of environmental fluoride via an engineered cell-free riboswitch-based biosensor” ACS Synthetic Biology 9(1): 10-18
107.
Hammerling MJ, Krüger A, Jewett MC (2020) “Strategies for in vitro engineering of the translation machinery” Nucleic Acids Research 48(3): 1068-1083
106.
Techner JM, Kightlinger W, Lin L, Hershewe J, Ramesh A, DeLisa MP, Jewett MC, Mrksich M (2019) “High-throughput synthesis and analysis of intact glycoproteins using SAMDI-MS” Analytical Chemistry 92(2): 1963-1971
105.
Kay JE, Jewett MC (2019) “A cell-free system for production of 2,3-butanediol is robust to growth-toxic compounds” Metabolic Engineering Communications 10: e00114
104.
Meyer A, Saaem I, Silverman AD, Varaljay V, Mikol R, Blum S, Onderko E, Bristol C, Liu S, Pratt K, Casini A, Eluere R, Moser F, Servinksy M, Lucks JB, Lux M, Glaven S, Crookes-Goodson W, Jewett MC, Gordon DB, Voigt CA (2019) “Organism engineering for the bioproduction of the triaminotrinitrobenzene (TATB) precursor phloroglucinol (PG)” ACS Synthetic Biology 8(12): 2746-2755
103.
Karim AS, Rasor BJ, and Jewett MC (2019) “Enhancing control of cell-free metabolism through pH modulation” Synthetic Biology 5(1): ysz027
102.
Kightlinger W, Duncker K, Ramesh A, Thames A, Natarajan A, Stark JC, Yang A, Lin L, Mrksich M, DeLisa MP, Jewett MC (2019) “A cell-free biosynthesis platform for modular construction of protein glycosylation pathways” Nature Communications 10: 5404
101.
Lee J, Schwieter KE, Watkins AM, Kim DS, Yu H, Schwarz KJ, Lim J, Coronado J, Byrom M, Anslyn EV, Ellington AD, Moore JS, Jewett MC (2019) “Expanding the limits of the second genetic code with ribozymes” Nature Communications 10: 5097
100.
Des Soye BJ, Gerbasi VR, Thomas PM, Kelleher NL, Jewett MC (2019) “A highly productive, one-pot cell-free protein synthesis platform based on genomically recoded Escherichia coli” Cell Chemical Biology 26: 1-12
99.
McNerney MP, Zhang Y, Steppe P, Silverman AD, Jewett MC, Styczynski MP (2019) “Point-of-care biomarker quantification enabled by sample-specific calibration” Science Advances 5 (9): eaax4473
98.
Carlson ED, d’Aquino AE, Kim DS, Fulk EM, Hoang K, Szal T, Mankin AS, Jewett MC (2019) “Engineered ribosomes with tethered subunits for expanding biological function” Nature Communications (10): 3920
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Yesselman JD, Eiler D, Carlson ED, Gotrik MR, d’Aquino AE, Ooms AN, Kladwang W, Carlson PD, Shi X, Costantino DA, Herschlag D, Lucks JB, Jewett MC, Kieft JS, Das R (2019) “Computational design of three-dimensional RNA structure and function” Nature Nanotechnology 14: 866-873
96.
Stark JC, Huang A, Hsu KJ, Dubner RS, Forbrook J, Marshalla S, Rodriguez F, Washington M, Rybnicky GA, Nguyen PQ, Hasselbacher B, Jabri R, Kamran R, Koralewski V, Wightkin W, Martinez T, Jewett MC (2019) “BioBits Health: Exploring engineering, biology, and human health with fluorescent readouts” ACS Synthetic Biology 8 (5):1001-1009
95.
O’Kane PT, Dudley QM, McMillan AK, Jewett MC, Mrksich M (2019) “High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA” Science Advances 5(6): eaaw9180
94.
Aleksashin NA, Leppik M, Hockenberry AJ, Klepacki D, Vázquez-Laslop N, Jewett MC, Remme J, Mankin AS (2019) “Assembly and functionality of the ribosome with tethered subunits” Nature Communications 10: 930
93.
Levine MZ, Gregorio NE, Jewett MC, Watts KR, Oza JP (2019) “Escherichia coli-based cell-free protein synthesis: protocols for a robust, flexible, and accessible platform technology” J. Vis. Exp. (144): e58882
92.
Dudley QM, Nash CJ, Jewett MC (2019) “Cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates” Synthetic Biology 4 (1): ysz003
91.
Silverman AD, Kelley-Loughnane N, Lucks JB, Jewett MC (2018) “Deconstructing cell-free extract preparation for in vitro activation of transcriptional genetic circuitry” ACS Synthetic Biology 8 (2): 403-414
90.
Quillin SJ, Hockenberry AJ, Jewett MC, Seifert HS (2018) “Neisseria gonorrhoeae exposed to sublethal levels of hydrogen peroxide mounts a complex transcriptional response” mSystems 3 (5): e00156-18
89.
Caschera F, Karim AS, Gazzola G, d’Aquino AE, Packard NH, Jewett MC (2018) “High-throughput optimization cycle of a cell-free ribosome assembly and protein synthesis system” ACS Synthetic Biology 7 (12): 2841-2853
88.
Bundy BC, Hunt JP, Jewett MC, Swartz JR, Wood DW, Frey DD, Rao G (2018) “Cell-free biomanufacturing” Current Opinion in Chemical Engineering 22: 177-183
87.
Des Soye BJ, Davidson SR, Weinstock MT, Gibson DG, Jewett MC (2018) “Establishing a high-yielding cell-free protein synthesis platform derived from Vibrio natriegens” ACS Synthetic Biology 7(9): 2245-2255
86.
Hockenberry AJ, Jewett MC, Amaral LAN, Wilke CO (2018) “Within-gene Shine-Dalgarno sequences are not selected for function” Molecular Biology and Evolution: msy150
85.
Huang A, Nguyen PQ, Stark JC, Takahasi MK, Donghia N, Ferrante T, Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ (2018) “BioBits™ Explorer: A modular synthetic biology education kit” Science Advances 4: eaat5105
84.
Stark JC, Huang A, Nguyen PQ, Dubner RS, Hsu KJ, Ferrante T, Anderson M, Kanapskyte A, Mucha Q, Packett JS, Patel P, Patel R, Qaq D, Zondor T, Burke J, Martinez T, Miller-Berry A, Puppala A, Reichert K, Schmid M, Brand L, Hill LR, Chellaswamy JF, Faheem N, Fetherling S, Gong E, Gonzalzles EM, Granito T, Koritsaris J, Nguyen B, Ottman S, Palffy C, Patel A, Skweres S, Slaton A, Woods T, Donghia N, Pardee K, Collins JJ, Jewett MC (2018) “BioBits™ Bright: A fluorescent synthetic biology education kit” Science Advances 4(8): eaat5107
83.
Jaroentomeechai T, Stark JC, Natarajan A, Glasscock C, Yates L, Hsu KJ, Mrksich M, Jewett MC, DeLisa MP (2018) “Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery” Nature Communications 9: 2686
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JA Schoborg, MC Jewett (2018) “Cell‐free protein synthesis: An emerging technology for understanding, harnessing, and expanding the capabilities of biological systems” Synthetic Biology: Parts, Devices and Applications, 301-330
81.
Karim AS, Jewett MC (2018) “Cell-free synthetic biology for pathway prototyping” Methods in Enzymology 608: 31-57
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d’Aquino AE, Kim DS, Jewett MC (2018) “Engineered ribosomes for basic science and synthetic biology” Annual Review of Chemical and Biomolecular Engineering 9: 311-340
79.
Wang H, Li J, and Jewett MC (2018) “Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements” Synthetic Biology 3(1): ysy003
78.
Casini A, Chang F-Y, Eluere R, King A, Young E, Dudley QM, Karim A, Pratt K, Bristol C, Forget A, Ghodasara A, Warden-Rothman R, Gan R, Cristofaro A, Borujeni AE, Ryu M, Li J, Kwon YC, Wang H, Tatsis E, Rodriguez-Lopez C, O’Connor S, Medema MH, Fischbach MA, Jewett MC, Voigt C, Gordon DB (2018) “A pressure test to make 10 molecules in 90 days: External evaluation of methods to engineer biology” J. Am. Chem. Soc. 140 (12): 4302-4316
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Kightlinger W, Lin L, Rosztoczy M, DeLisa MP, Mrksich M, Jewett MC (2018) “Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases” Nature Chemical Biology 14: 627-635
76.
Martin RW, Des Soye BJ, Kwon YC, Kay J, Davis RG, Thomas PM, Marcum RD, Majewska NI, Chen CX, Weiss MG, Stoddart AE, Amiram M, Charna AK, Isaacs FJ, Kelleher NL, Hong SH, Jewett MC (2018) “Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids” Nature Communications 9: 1203
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Kakkar N, Perez JG, Liu W, Jewett MC, van der Donk W (2018) “Incorporation of non-proteinogenic amino acids in class I and II lantibiotics” ACS Chemical Biology 13: 951-957
74.
Aebersold R, Agar JN, Amster J, Baker M, Bertozzi CR, Boja ES, Costello CE, Cravatt BF, Fenselau C, Garcia B, Ge Y, Gunawardena J, Hendrickson RC, Hergenrother PJ, Huber C, Ivanov AR, Jensen O, Jewett MC, Kelleher NL, Kiessling LL, Krogan NJ, Larsen MR, Loo JA, Loo RR, Lundberg E, MacCoss M, Mallick P, Mootha VK, Mrksich M, Muir TW, Patrie SM, Pesavento JJ, Pitteri SJ, Rodriguez H, Saghatelian A, Sandoval W, Schlüter H, Sechi A, Sheeley DM, Slavoff SA, Smith LM, Snyder MP, Thomas PM, Uhlen M, van der Donk WA, van Eyk JE, Vidal M, Walt DR, White FM, Williams ER, Wohlschlager T, Wysocki VH, Yates NA, Young NL, Zhang B (2018) “How many human proteoforms are there?” Nature Chemical Biology 14(3): 206-214
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Liu SS, Hockenberry AJ, Lancichinetti A, Jewett MC, Amaral LAN (2018) “A novel framework for evaluating the performance of codon usage bias metrics” Journal of the Royal Society Interface 15(138): 20170667
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Liu CC, Jewett MC, Chin JW, Voigt CA (2018) “Towards an orthogonal central dogma” Nature Chemical Biology 14:103-106
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Schoborg JA, Hershewe J, Stark JC, Kightlinger W, Kath JE, Jaroentomeechai T, Natarajan A, DeLisa MP, Jewett MC (2018) “A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases ” Biotechnology and Bioengineering 115(3): 739-750
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Li J, Wang H, Jewett MC (2018) “Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields” Biochemical Engineering Journal 130: 29-33
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Hockenberry AJ, Stern AJ, Amaral LAN, Jewett MC (2018) “Diversity of translation initiation mechanisms across bacterial species is driven by environmental conditions and growth demands” Molecular Biology and Evolution 35 (3): 582-592
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Karim AS, Heggestad JT, Crowe SA, Jewett MC (2018) “Controlling cell-free metabolism through physiochemical perturbations” Metabolic Engineering 45: 86-94
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Martin RW, Majewska NI, Chen CX, Albanetti TE, Jimenez RB, Schmelzer AE, Jewett MC, Roy V (2017) “Development of a CHO-based cell-free platform for synthesis of active monoclonal antibodies” ACS Synthetic Biology 6(7): 1370-1379
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Li J, Haas W, Jackson K, Kuru E, Jewett MC, Fan ZH, Gygi SP, and Church GM (2017) “Co-generating synthetic parts toward a self-replicating system” ACS Synthetic Biology 6(7): 1370-1379
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Jaroentomeechai T, Zheng X, Hershewe J, Stark JC, Jewett MC, and DeLisa MP (2017) “A pipeline for studying and engineering single-subunit oligosaccharyltransferases” Methods in Enzymology, Chemical Glycobiology 597: 55-81
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Liu Y, Kim DS, Jewett MC (2017) “Repurposing ribosomes for synthetic biology” Current Opinion in Chemical Biology 40: 87-94
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Li J, Wang H, Kwon YC, Jewett MC (2017) “Establishing a high yielding Streptomyces-based cell-free protein synthesis system” Biotechnology and Bioengineering 114(6): 1343-1353
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Hockenberry AJ, Pah AR, Jewett MC, Amaral LAN (2017) “Leveraging genome-wide datasets to quantify the functional role of the anti-Shine-Dalgarno sequence in regulating translation efficiency” Open Biology 7(1): 160239
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Gan R, Perez JG, Carlson ED, Ntai I, Isaacs FJ, Kelleher NL, Jewett MC (2017) “Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins” Biotechnology and Bioengineering 114(5): 1074-1086
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Liu SS, Hockenberry AJ, Jewett MC, Amaral LAN (2016) “NullSeq: A tool for generating random coding sequences with desired amino acid and GC contents” PLoS Computational Biology 12(11): e1005184
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Yang C, Hockenberry AJ, Jewett MC, Amaral LAN (2016) “Depletion of Shine-Dalgarno sequences within bacterial coding regions is expression dependent” G3: Genes|Genomes|Genetics 6(11): 3467-3474
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Goering AW, Li J, McClure RA, Thomson RJ, Jewett MC, Kelleher NL (2016) “In vitro reconstruction of nonribosomal peptide biosynthesis directly from DNA using cell-free protein synthesis” ACS Synthetic Biology 6: 39-44
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Dudley QM, Anderson KC, Jewett MC (2016) “Cell-free mixing of Escherichia coli crude extracts to prototype and rationally engineer high-titer mevalonate synthesis” ACS Synthetic Biology 5(12): 1578-1588
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Karim AS, Jewett MC (2016) “A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery” Metabolic Engineering 36: 116-126
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Schoborg JA, Clark LG, Choudhury A, Hodgman CE, Jewett MC (2016) “Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis” Synthetic and Systems Biotechnology 1(1): 2-6
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Caschera F, Lee JW, Ho KKY, Liu AP, Jewett MC (2016) “Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes” Chemical Communications 52(31): 5467-5469
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Gan R, Jewett MC (2016) “Evolution of translation initiation sequences using in vitro yeast ribosome display” Biotechnology and Bioengineering 113:1777-1786
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Li J, Lawton TJ, Kostecki JS, Nisthal A, Fang J, Mayo SL, Rosenzweig AC, Jewett, MC (2016) “Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase” Biotechnology Journal 11: 212–218
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Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW, Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Söll D, Rinehart J, Isaacs FJ (2015) “Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids” Nature Biotechnology 33: 1272-1279
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Sullivan CJ, Pendleton ED, Sasmor HH, Hicks WL, Farnum JB, Muto M, Amendt EM, Schoborg JA, Martin RW, Clark LG, Anderson MJ, Choudhury A, Fior R, Lo YH, Griffey RH, Chappell SA, Jewett MC, Mauro VP, Dresios J (2016) “A cell-free expression and purification process for rapid production of protein biologics” Biotechnology Journal 11: 238-248
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Kay JE, Jewett MC (2015) “Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2, 3-butanediol” Metabolic Engineering 32: 133-142
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Des Soye B, Patel J, Isaacs FJ, Jewett MC (2015) “Repurposing the translation apparatus for synthetic biology” Current Opinion in Chemical Biology 28: 83-90
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Oza JP, Aerni HR, Pirman NL, Barber KW, ter Haar CM, Rogulina S, Amrofell MB, Isaacs FJ, Rinehart J, Jewett MC (2015) “Robust production of recombinant phosphoproteins using cell-free protein synthesis” Nature Communications 6: 8168
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Orelle C, Carlson ED, Szal T, Florin T, Jewett MC, Mankin AS (2015) “Protein synthesis by ribosomes with tethered subunits” Nature 524: 119–124
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Anderson MJ, Stark JC, Hodgman CE, Jewett MC (2015) “Energizing yeast cell-free protein synthesis with glucose metabolism” FEBS Letters 589: 1723-1727
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Moatsou D, Li J, Ranji A, Pitto-Barry A, Ntai I, Jewett MC, O’Reilly RK (2015) “Reversible self-assembly of temperature-responsive protein-polymer conjugates” Bioconjugate Chemistry 26: 1890-1899
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Fritz BR, Jamil OK, and Jewett MC (2015) “Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction” Nucleic Acids Research 43: 4774-4784
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Heinzelman P, Schoborg JA, Jewett MC (2015) “pH responsive granulocyte colony stimulating factor variants with implications for treating Alzheimer’s disease and other central nervous system disorders” Protein Engineering Design and Selection 28: 481-489
41.
Hong SH, Kwon YC, Martin RW, Des Soye BJ, de Paz AM, Swonger KN, Ntai I, Kelleher NL, Jewett MC (2015) “Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking Release Factor 1″ ChemBioChem 16: 844-853 (inner cover)
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Kwon YC, Jewett MC (2015) “High-throughput preparation methods of crude extract for robust cell-free protein synthesis” Scientific Reports 5: 8663
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Ploskon E, Wagner S, Ellington AD, Jewett MC, O’Reilly R, Booth P (2015) “Controlled assembly of artificial protein-protein complexes via DNA duplex formation” Bioconjugate Chemistry 26: 427-434
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Dudley QM, Karim AS, Jewett MC (2015) “Cell-free metabolic engineering: Biomanufacturing beyond the cell” Biotechnology Journal 10: 69-82
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Choudury A, Hodgman CE, Anderson M, Jewett MC (2014) “Evaluating fermentation effects on cell growth and crude extract metabolic activity for improved yeast cell-free protein synthesis” Biochemical Engineering Journal 91: 140-148
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Hodgman CE, Jewett MC (2014) “Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis” New Biotechnology 31: 499-505
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Liu Y, Fritz BR, Anderson MJ, Schoborg JA, Jewett MC (2014) “Characterizing and alleviating substrate limitations for improved in vitro ribosome construction” ACS Synthetic Biology 4: 454-462
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Fritz BR, Jewett MC (2014) “The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction” Nucleic Acids Research 42: 6774-6785
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Gan R, Jewett MC (2014) “A combined cell-free transcription-translation system from Saccharomyces cerevisiae for rapid and robust protein synthesis” Biotechnology Journal 9: 641-651
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Schoborg JA, Hodgman CE, Anderson M, Jewett MC (2014) “Characterizing substrate limitations for improved yeast cell-free protein synthesis” Biotechnology Journal 9: 631-640
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Hockenberry AJ, Sirer MA, Amaral LAN, Jewett MC (2014) “Quantifying position dependent codon usage bias” Molecular Biology and Evolution 31: 1880-1893
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Palmer MJ, Jewett MC (2014) “Enabling a next generation of synthetic biology community organization and leadership” ACS Synthetic Biology 3: 117-120 (Viewpoint)
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Hong SH, Ntai I, Haimovich AD, Kelleher NL, Isaacs FJ, Jewett MC (2014) “Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific non-standard amino acid incorporation” ACS Synthetic Biology 3: 398-409
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Hong SH, Kwon YC, Jewett MC (2014) “Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis” Frontiers in Chemistry 2: 34
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Jewett MC, Workman CT, Nookaew I, Pizarro FP, Hellgren L, Nielsen J (2013) “Mapping condition
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Hodgman CE, Jewett MC (2013) “Optimizing extract preparation and reaction conditions to increase yield and reduce costs in a yeast cell-free protein synthesis system” Biotechnology and Bioengineering 110: 2643-2654
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Jewett MC, Patolsky F (2013) “Nanobiotechnology: Synthetic biology meets materials science” Current Opinion in Biotechnology 24: 551-554 (Editorial Overview)
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Kang Y, Lu A, Ellington AD, Jewett MC, O’Reilly RK (2013) “Effect of complementary nucleobase interactions on the copolymer composition of RAFT copolymerizations” ACS Macro Letters 2: 581-586
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Jewett MC, Fritz BR, Timmerman LE, Church GM (2013) “In vitro integration of ribosomal RNA synthesis, ribosome assembly, and protein synthesis” Molecular Systems Biology 9: 678
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Harris DC, Jewett MC (2012) “Cell-free biology: Exploiting the interface between synthetic biology and synthetic chemistry” Current Opinion in Biotechnology 23: 672-678
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Hockenberry AJ, Jewett MC (2012) “Synthetic in vitro circuits” Current Opinion in Chemical Biology 16: 261-269
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Hodgman CE, Jewett MC (2012) “Cell-free synthetic biology: Thinking outside the cell” Metabolic Engineering 14: 261-269
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Carlson ED, Gan R, Hodgman CE, Jewett MC (2011) “Cell-free protein synthesis: Applications come of age” Biotechnology Advances 30: 1185-1194
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Wang HH, Huang P-Y, Xu G, Haas W, Marblestone A, Li J, Gygi S, Forster AC, Jewett MC, Church GM (2012) “In vivo tagging of enzyme ensembles with MAGE for in vitro single-pot multi-enzyme catalysis” ACS Synthetic Biology 1: 43-52
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Isaacs FJ, Carr PA, Wang HH, Lajoie MJ, Sterling B, Kraal L, Tolonen A, Gianoulis T, Goodman D, Reppas NB, Emig CJ, Bang D, Hwang SJ, Jewett MC, Jacobson JM, Church GM (2011) “Precise manipulation of chromosomes in vivo enables genome-wide codon replacement” Science 333: 348-353
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Fritz BR, Timmerman LE, Daringer NM, Leonard JN, Jewett MC (2010) “Biology by design: From top to bottom and back” Journal of Biomedicine and Biotechnology 2010: 11, Article ID 232016
15.
Jewett MC, Forster AC (2010) “Update on designing and building minimal cells” Current Opinion in Biotechnology 21: 697-703
14.
Moxley JF, Jewett MC, Antoniewicz MR, Villas-Boas SG, Alper HS, Wheeler RT, Tong L, Hinnebusch AG, Ideker T, Nielsen J, Stephanopoulos GN (2009) “Linking high resolution metabolic flux phenotypes and transcriptional regulation in yeast modulated by the global regulator Gcn4p” PNAS 106: 6477-6482
13.
Jewett MC, Miller ML, Chen Y, Swartz JR (2009) “Continued protein synthesis at low [ATP] and [GTP] enables cell adaptation during starvation” Journal of Bacteriology 191: 1083-1091
12.
Usaite R, Jewett MC, Oliveira AP, Yates JR, Olsson L, Nielsen J (2009) “Reconstruction of the yeast Snf1 kinase regulatory network reveals its role as a global energy regulator” Molecular Systems Biology 5: 319
11.
Pizarro FJ, Jewett MC, Nielsen J, Agosin E (2008) “Physiological and transcriptional mapping of evolutionary differences between commercial and laboratory Saccharomyces cerevisiae strains” Applied Environmental Microbiology 74: 6358-6368
10.
Nookaew N, Jewett MC, Meechai A, Thammarongtham C, Laoteng K, Cheevadhanarak S, Nielsen J, Bhumiratana S (2008) “The genome-scale metabolic model iIN800 of Saccharomyces cerevisiae and its validation: A scaffold to query lipid metabolism” BMC Systems Biology 2: 71
9.
Fazio A, Jewett MC, Daran-Lapujade P, Mustacchi R, Usaite R, Pronk JT, Workman CT, Nielsen J (2008) “Transcription factor control of growth rate dependent genes in Saccharomyces cerevisiae: A three factor design” BMC Genomics 9: 341
8.
Nielsen J, Jewett MC (2008) “The impact of systems biology and metabolic engineering of Saccharomyces cerevisiae” FEMS Yeast Research 8: 122-31
7.
Jewett MC, Calhoun KA, Voloshin A, Wuu JJ, Swartz JR (2008) “An integrated cell-free metabolic platform for protein production and synthetic biology” Molecular Systems Biology 4: 220
6.
Jewett MC, Hofmann G, Nielsen J (2006) “Fungal metabolite analysis in genomics and phenomics” Current Opinion Biotechnology 17: 191-197
5.
Jewett MC, Oliveira AP, Patil KR, Nielsen J (2005) “The role of high-throughput transcriptome analysis in metabolic engineering” Biotechnology and Bioprocess Engineering 10: 385-399
4.
Jewett MC, Swartz JR (2004) “Substrate replenishment extends protein synthesis with an in vitro system designed to mimic the cytoplasm” Biotechnology and Bioengineering 87: 465-472
3.
Jewett MC, Swartz JR (2004) “Mimicking the Escherichia colicytoplasmic environment activates long-lived and efficient protein synthesis” Biotechnology and Bioengineering 86: 19-26
2.
JR Swartz, MC Jewett, KA Woodrow (2004) “Cell-free protein synthesis with prokaryotic combined transcription-translation” Recombinant Gene Expression 267: 169-182
1.
Jewett MC, ad Swartz JR (2004) “Rapid expression and purification of 100 nmol quantities of active protein using cell-free protein synthesis” Biotechnology Progress 20: 102-109
9.
Karim AS, Dudley QM, and Jewett MC (2017) “Cell-free synthetic systems for metabolic engineering and biosynthetic pathway prototyping,” in Industrial Biotechnology, James C. Liao, editor. Wiley Biotechnology Series: 125-148
8.
Perez JG, Stark JC, Jewett MC (2016). “Cell-free synthetic biology: Engineering beyond the cell,” in Synthetic Biology, J. Craig Venter, Daniel Gibson, Hamilton Smith, Clyde Hutchison, editors. Cold Spring Harbor Laboratory Press: a023853
7.
Schoborg JA, Jewett MC (2015) “Cell-free protein synthesis: An emerging technology for understanding, harnessing, and expanding the capabilities of biological systems,” in Synthetic Biology, Sven Panke and Christina Smolke, editors. Wiley Biotechnology Series: 125-148
6.
Ranji A, Wu JC, Bundy BC, Jewett MC (2013) “Transforming synthetic biology with cell-free systems,” in Synthetic Biology, Huimin Zhao, editor. Waltham, MA, Elsevier: 277-301
5.
Oliveira AP, Jewett MC, Nielsen J (2007) “From gene expression to metabolic fluxes” in Introduction to Systems Biology, Sangdun Choi, editor. Totowa, NJ, Humana Press Inc: 37-68
4.
Nielsen J, Jewett MC (2007) “The role of metabolomics in systems biology” in Metabolomics: A powerful tool in systems biology, Jens Nielsen, Michael Jewett, editors. Topics in Current Genetics Series. Heidelberg, Germany, Springer: 1-10
3.
Jewett MC, Hansen ME, Nielsen J (2007) “Data acquisition, analysis, and mining: Integrative tools for discerning metabolic function in Saccharomyces cerevisiae” in Metabolomics: A powerful tool in systems biology, Jens Nielsen, Michael Jewett, editors. Topics in Current Genetics Series. Heidelberg, Germany, Springer: 159-187.
2.
Swartz JR, Jewett MC, Woodrow KA (2004) “Cell-free protein synthesis with prokaryotic combined transcription-translation” in Recombinant Protein Protocols, Paulina Balbas, Argelia Lorence, editors. Methods in Molecular Biology Series, Vol. 267. Totowa, NJ, Humana Press Inc: 169-182
1.
Jewett MC, Voloshin A, and Swartz JR (2002) “Prokaryotic systems for in vitro expression” in Gene Cloning and Expression Technologies”, Michael Weiner, Quinn Lu, editors. Westborough, MA, Eaton Publishing: 391-411
10.
Swartz JR, Jewett MC (2013) Improved methods of in vitro protein synthesis. Canadian Patent No. 2,496,437
9.
Calhoun KA, Jewett MC, and Swartz JR (2013) Improved methods of in vitro protein synthesis. Korean Patent No. 10-1232656
8.
Swartz JR, Jewett MC (2013) Methods of in vitro protein synthesis. U.S. Patent No. 8,357,529
7.
Bond R, Jewett MC, McAuliffe JC, and Ward DE (2010) Biocomposite comprising co-precipitate of enzyme, silicate and polyamine. U.S. Patent No. 7,642,077
6.
Swartz JR, Jewett MC (2010) A method for synthesis of polynucleotides and/or polypeptides. Indian Patent No. 239129
5.
Calhoun KA, Jewett MC, Swartz JR (2009) Improved methods of in vitro protein synthesis. Australian Patent No. 2004293798
4.
Swartz JR, Jewett MC (2009) Improved methods of in vitro protein synthesis. Australian Patent No. 2003259912
3.
Calhoun KA, Jewett MC, Swartz JR (2009) Improved methods of in vitro protein synthesis. New Zealand Patent No. 546961
2.
Swartz JR, Jewett MC (2009) Improved methods of in vitro protein synthesis. European Patent No. 1539948.