Publications

Publication-Covers

Google Scholar Citations:

Disclaimer: The following PDFs are intended for personal use. All persons reproducing, redistributing, or making commercial use of this information are expected to adhere to the terms and conditions as asserted by the copyright holder. Transmission or reproduction of protected items beyond that are allowed by fair use as defined in the copyright laws requires the written permission of the copyright owners.

2024

217

Landwehr, G.M., Vogeli, B., Tian, C., Singal, B., Gupta, A., Lion, R., Sargent, E.H., Karim, A.S.,* and Jewett, M.C.* A synthetic cell-free pathway for biocatalytic upgrading of one-carbon substrates. Biorxiv. in press

216

Sotomayor, B., Donahue, T.C., Mahajan, S.P., Taw, M.N., Hulbert, S.W., Bidstrup, E.J., Pang, A., Yang, X., Azadi, P., Gray, J.J., Jewett, M.C., Wang, L-X., and DeLisa, M.P.* Glycosylation of full-length IgG antibodies in bacteria enabled by a single-subunit oligosaccharyltransferase from Desulfovibrio marinus. Biorxiv in press

215

Landwehr, G.M.,† Bogart, J.W.,† Magalhaes, C.,% Hammarlund, E.G., A. Karim, A.S.,* and Jewett, M.C.* Accelerated enzyme engineering by machine-learning guided cell-free expression. Biorxiv in press

214

Wong, D.A., Cabezas, M.D., Daniel-Ivad, M., Prasanna, D.V., Fernandez, R.,% Nicol, R., Balskus, E.P., Karim, A.S.* and Jewett, M.C.* A cell-free workflow for detecting and characterizing RiPP recognition element-precursor peptide interactions. Biorxiv. in press

213

Ekas, H.M.,† Wang, B.,† Silverman, A.D., Lucks, J.B., Karim, A.S., and Jewett, M.C.* Engineering a PbrR-based biosensor for cell-free detection of lead at the legal limit. ACS Synthetic Biology.

212

Alejo, J.L.,† Girodat, D.,† Hammerling, M.J., Willi, J.A., Jewett, M.C., Engelhart, A.E., and Adamala, K.P. Alternate conformational trajectories in protein synthesis. PLOS Computational Biology. 20: e1012319.

211

Willi, J.A., Karim, A.S., and Jewett, M.C.* 2024. Cell-free translation quantification via a fluorescent minihelix. ACS Synthetic Biology. 13: 2253–2259.

210

Collins, M.C.,† Lau, M.,† Ma, W., Shen, A., Wang, B.W., Cai, S, Russa, M.L., Jewett, M.C., and Qi, L.S.* CRISPRkit: A frugal gene editing and regulation kit for equitable and accessible education. Nature Communications. 15: 6563.

209

Palma, J., Bunyatov, M., Hulbert, S., Jewett, M.C., and DeLisa, M.P.* 2024. Bacterial glycoengineering: cell based and cell-free routes for producing biopharmaceuticals with customized glycosylation. Current Opinion in Chemical Biology. 81: 10250.

208

DeWinter, M.A.,† Wong, D.A., Fernandez, R.,% Kightlinger, W., Thames, A.H., DeLisa, M.P., and Jewett, M.C.* 2024. Establishing a cell-free glycoprotein synthesis system for enzymatic N-GlcNAcylation. ACS Chemical Biology. 19: 1570–1582.

207

Liu, W-Q., Ji, X., Ba, F., Zhang, Y., Xu, H., Huang, S., Zheng, X., Liu, Y., Ling, S., Jewett, M.C, and Li., J. 2024. Cell-free biosynthesis and engineering of ribosomally synthesized lanthipeptides. Nature Communications. 15: 4336.

206

Clark, L., Voigt, C.A., and Jewett, M.C.* 2024. Establishing a high yielding chloroplast cell-free system for prototyping genetic parts. ACS Synthetic Biology.

205

Böhm, C.V.,† Inckemann, R.,† Burgis, M.,% Baumann, J.,% Brinkmann, C.K.,% Lipińska, K.E.,% Gilles, S.,% Freudigmann, J., Seiler, V., Clark, L.G., Jewett, M.C., Voll, L.M.,* and Niederholtmeyer, H.* 2024. Chloroplasts cell-free systems as rapid prototyping platforms for high-throughput part characterization. ACS Synthetic Biology.

204

Karim, A.S.,* Brown, D.M., Archuleta, C.M., Grannan, S., Aristilde, L., Goyal, Y., Leonard, J.N., , Niall Mangan, N., Prindle, A., Rocklin, G.J., Tyo, K.J., Zoloth, L., Jewett, M.C., Calkins, S., Kamat, N.,Tullman-Ercek, D., and Lucks, J.B.* 2024. Deconstructing synthetic biology across scales: a conceptual approach for training synthetic biologists. Nature Communications. 15: 5425.

203

Jensen, R.O.,† Schulz, F.,† Roux, S., Klingeman, D.M., Reynoso, V., Winkler, J., Nagaraju, S., Mitchell, W.P., Moraïs, S., Tissera, S.D., Shapiro, N., Ivanova, N., Reddy, T.B.K., Mizrahi, I., Utturkar, S., Bayer, E.A., Woyke, T., Mouncey, N.J., , Jewett, M.C., Simpson, S.D., Köpke, M., Jones, D.T.,, and Brown, S.D. 2024. Phylogenomics and genetic analysis of solvent-producing Clostridium species. Scientific Data. 11: 432.

202

Kofman, C., Willi, J.A., Karim, A.S., and Jewett, M.C.* 2024. Ribosome pool engineering increases protein biosynthesis yields. ACS Central Science. 10:871–881. (Highlighted in C&E News).

201

Lin, L., Kightlinger, W., Warfel, K., Jewett, M.C.,* and Mrksich, M.* 2024. Using high- throughput experiments to engineer N-glycosyltransferases with altered specificities. ACS Synthetic Biology. 13:1290-1302. in press

200

Rothschild, L.J.,* Averesch, Nils, J.H., Strychalski, E., Moser, F., Glass, J.I., Perez, R.C., Yekinni, I.O., Rothschild-Mancinelli, B., Roberts Kingman, G.A., Wu, F., Waeterschoot, J., Ioannou, I.A., Jewett, M.C., Liu, A.P., Noireaux, V., Sorenson, C., and Adamala, K.P. 2024. Building synthetic cells – from the technology infrastructure to cellular entities. ACS Synthetic Biology. 13:974-997. (Highlighted by NASA).

199

Kocalar, S., Miller, B.M., Huang, A., Gleason, E., Martin, K., Foley, K., Copeland, D.S., Jewett, M.C., Saavedra, E.A., and Kraves, S.* 2024. Validation of cell-free protein synthesis aboard the International Space Station. ACS Synthetic Biology. 13: 942–950.

198

Steinküher, J.,† Peruzzi, J.A.,† Krüger, K., Jacobs, M.L., Jewett, M.C., and Kamat, N.P.* 2024. Improving cell-free expression of membrane proteins by tuning ribosome co-translational membrane association and nascent chain aggregation. ACS Synthetic Biology. 13: 29–140.

2023

197

Yia, X.,† Rasor, B.J.,†, Boadi, N.,% Louie, K., Northern, T.R., Karim, A.S., Jewett, M.C.,* and Alper, H.A.* 2023. Establishing a versatile toolkit of flux enhanced strains and cell extracts for pathway prototyping. Metabolic Engineering. 80:241-253.

196

Hulbert, S.W., Desai, P., Jewett, M.C., DeLisa, M.P.,* Williams, A.J.* 2023. Glycovaccinology: The design and engineering of carbohydrate-based vaccine components. Biotechnology Advances. 108234.

195.

Hulbert, S.W., Desai, P., Jewett, M.C., DeLisa, M.P.,* Williams, A.J.* 2023. “Glycovaccinology: The design and engineering of carbohydrate-based vaccine components.” Biotechnology Advances.

194.

Thames, A.H. Rische, C.H., Yun Cao, Y., Krier-Burris, R.A., Kuang, F.L., Hamilton, R.G., Bronzert, C,. Bochner, B.S.,* and Jewett, M.C.* 2023. “A cell- free protein synthesis platform to produce a clinically relevant allergen panel” ACS Synthetic Biology. 2252–2261

193.

Steinkuher J, Peruzzi JA, Kruger K, Jacobs ML, Jewett MC (2023) “Improving cell-free expression of membrane proteins by tuning ribosome co-translational membrane association and nascent chain aggregation” ACS Syntethic Biology

192.

Jung KJ, Rasor BJ, Rybnicky GA, Silverman AD, Standevene J, Kuhn R, Granitoh T, Ekas HM, Wang BM, Karim AS, Lucks JB, Jewett MC (2023) “At-home, cell-free synthetic biology education modules for transcriptional regulation and environmental water quality monitoring” ACS Synthetic Biology

191.

Thames AH, Moons SJ, Wong DA, Boltje TJ, Bochner BS, Jewett MC (2023) “GlycoCAP: A cell-free, bacterial glycosylation platform for building clickable azido-sialoglycoproteins” ACS Synthetic Biology

190.

Seki K, Galindo JL, Karim AS, Jewett MC (2023) “A cell-free gene expression platform for discovering and characterizing stop codon suppressing tRNAs” ACS Chemical Biology

189.

Hunt AC, Vogeli B, Kightlinger WK, Yoesep DJ, Kruger A, Jewett MC (2023) “A high-throughput, automated, cell-free expression and screening platform for antibody discovery” Nature Communications

188.

Lim J, Coronado JN, Hosford B, Roy NM, Martin C, Lee J, Jewett MC, Anslyn EV (2023) “Cyclocondensation reactions of a bifunctional monomer bearing a nucleophilic hydrazine and electrophilic ketoester pair” Tetrahedron Letters

187.

Stark JC, Jaroentomeechai T, Warfel KF, Hershewe JM, DeLisa MP, Jewett MC (2023) “Rapid biosynthesis of glycoprotein therapeutics and vaccines from freeze-dried bacterial cell lysates” Nature Protocols

186.

DeWinter MA, Thames AH, Guerrero L, Kightlinger WK, Karim AS, Jewett MC (2023) “Point-of-care peptide hormone enabled by cell-free protein synthesis” ACS Synthetic Biology

185.

Rasor BJ, Karim AS, Alper HA, Jewett MC (2023) “Cell extracts from bacteria and yeast retain metabolic activity after extended storage and repeated thawing” ACS Synthetic Biology

184.

Warfel KF, Laigre E, Sobol SE, Gillone E, Varrote A, Renaudetd O, Dejeud J, Jewett MC, Imbertye A (2023) “Cell-free expression and characterization of multivalent rhamnose-binding lectins using biolayer interferometry” Glycobiology

183.

Williams AJ, Warfel KT, Desai P, Li J, Jen-Jie Lee JJ, Wong DA, Sobel SE, Jewett MC, Chang YF, DeLisa MP (2023) “A low-cost recombinant glycoconjugate vaccine confers immunogenicity and protection against enterotoxigenic Escherichia coli infections in mice” Frontiers in Microbiology

182.

Kruger A, Watkins AM, Wellington-Oguri R, Romano J, Kofman C, DeFoe A, Kim Y, Anderson-Lee J, Fisker E, Townley J, Eterna participants, d’Aquino AE, Ras R, Jewett MC (2023) “Community science designed ribosomes with beneficial phenotypes” Nature Communications 14:961

181.

Rasor BJ, Chirania P, Rybnicky GA, Giannone RJ, Engle NL, Tschaplinski TJ, Karim AS, Hettich RL, Jewett MC (2023) “Mechanistic insights into cell-free gene expression through an integrated -omics analysis of extract processing methods” ACS Synthetic Biology 12(2): 405-418

180.

Martin J, Rasor B, DeBonis J, Karim AS, Jewett MC, Broadbelt LJ, Tyo KEJ (2023) “Dynamic kinetic models captures cell-free metabolism for improved butanol production” Metabolic Engineering 76:133-145

179.

Lee H, Willi JA, Cho N, Kim I, Jewett MC, Lee J (2023) “Cell-free biosynthesis of peptidomimetics” Biotechnology and Bioprocess Engineering /doi.org/10.1007/s12257-022-0268-5

178.

Warfel KT, Williams A, Wong DA, Sobol SE, Desaie P, Chang Y-F, Karim AS, DeLisa M, Jewett MC (2023) “A Low-Cost, Thermostable, Cell-Free Protein Synthesis Platform for On-Demand Production of Conjugate Vaccines” ACS Synthetic Biology 10

2022

177.

Chung SS, Bidstrup EJ, Hershewe JM, Warfel KF, Jewett MC, DeLisa MP (2022) “Ribosome stalling of N-linked glycoproteins in cell-free extracts” ACS Synthetic Biology 11: 3892–3899

176.

Koo C, Hershewe JM, Jewett MC, Rosenzweig AC (2022) “Cell-free protein synthesis of particulate methane monooxygenase into nanodiscs” ACS Synthetic Biology 11: 4009-4017

175.

Lee J, Coronado JN, Cho N, Lim J, Hosford BM, Seo S, Kim DS, Kofman C, Moore JS, Ellington AD, Anslyn EV, Jewett MC (2022) “Ribosome-mediated biosynthesis of pyridazinone oligomers in vitro” Nature Communications 13: 6322

174.

Nielsen MI, de Haan N, Kightlinger W, Dabelsteen S, Li M, Jewett MC, Bagdonaite I, Yakhrushev SY, Wandall HH (2022) “Global mapping of GalNAc-T isoform-specificities and O-glycosylation site-occupancy in a tissue-forming human cell line” Nature Communications 13: 6257

173.

Jaroentomeechai T, Liu Y, Young O, Bhawal R, Wilson JD, Li M, Chapla DG, Moremen KW, Jewett MC, Mizrachi D, DeLisa MP (2022) “A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans” Nature Communications 13: 6325

172.

Kofman C, Watkins AM, Kim DS, Wooldredge AC, Karim AS, Das D, Jewett MC (2022) “Computationally-guided design and selection of ribosomal active site mutants with high activity” Nucleic Acids Research 50: 13143-13154

171.

Nogle R, Nagaraju S, Utturkar SM, Giannone RJ, Reynoso V, Leang C, Hettich R, Mitchell WP, Simpson SD, Jewett MC, Kopke M, Brown S (2022) “Clostridium autoethanogenum isopropanol production via native plasmid pCA replicon” Frontiers in Bioengineering and Biotechnology 10

170.

Mills CE, Waltmann C, Archer A, Kennedy NW, Abrahamson CH, Jackson A, Shirman S, Jewett MC, Mangan NM, Olvera de la Cruz M, Tullman-Ercek D (2022) “Vertex protein PduN tunes encapsulated pathway performance by dictating bacterial metabolosome morphology” Nature Communications 13(3746)

169.

Harbaugh S, Silverman AD, Chushak Y, Zimlich K, Wolfe M, Thavarajah W, Jewett MC, Lucks JB, Chavez J (2022) “Engineering a synthetic dopamine-responsive riboswitch for in vitro biosensing” ACS Synthetic Biology 11(7): 2275–2283

168.

Schachner LF, Des Soye B, Ro S, Kenney GE, Ives AN, Su T, Goo YA, Jewett MC, Rosenzweig AC, Kelleher NL (2022) “Revving an Engine of Human Metabolism: Activity Enhancement of Triosephosphate Isomerase via Hemi-Phosphorylation” ACS Chemical Biology 17(10): 2769–2780

167.

Gowland S, Jewett MC (2022) “Mobile Translation Systems Generate Genomically Engineered Escherichia coli Cells with Improved Growth Phenotypes” ACS Synthetic Biology 11(9): 2969-2978

166.

Kim DS, Watkins A, Bidstrup E, Lee J, Topkar V, Kofman C, Schwarz KJ, Liu Y, Pintilie G, Roney E, Das R, Jewett MC (2022) “Three-dimensional structure-guided evolution of a ribosome with tethered subunits” Nature Chemical Biology 18: 990-998

165.

Charna AKR, Des Soye BJ, Ioanni Ntai I, Kelleher NL, Jewett MC (2022) “An efficient cell-free protein synthesis platform for the incorporation of pyrrolysine-based non-canonical amino acids” Biotechnology Journal 17(9)

164.

Agarwal DK, Hunt AC, Shekhawat GS, Carter L, Chan S, Wu K, Cao L, Baker D, Lorenzo-Redondo R, Ozer EA, Simons LM, Hultquist JF, Jewett MC, Dravid VP (2022) “Rapid and sensitive detection of antigen from SARS-CoV-2 variants of concern by a multivalent minibinder-functionalized nanomechanical sensor” Analytical Chemistry 94(23): 8105-8109

163.

Vogeli B, Schulz L, Garg S, Tarasava K, Clomburg JM, Lee SH, Gonnot A, Moully EH, Kimmel BR, Mrksich M, Karim AS, Gonzalez R, Kopke M, Jewett MC. (2022) “Cell-free prototyping enables implementation of optimized reverse b-oxidation pathways in heterotrophic and autotrophic bacteria” Nature Communications, 13(3058)

162.

Kennedy NW, Mills CE, Abrahamson CH, Archer AG, Shirman S, Jewett MC, Mangan NM, Tullman-Ercek D (2022) “Linking the Salmonella enterica 1,2-Propanediol Utilization Bacterial Microcompartment Shell to the Enzymatic Core via the Shell Protein PduB” Journal of Bacteriology 204(9)

161.

Gan R, Cabezas MD, Pan M, Zhang H, Hu G, Clark LG, Jewett MC, Nicol R (2022) “High-Throughput Regulatory Part Prototyping and Analysis by Cell-Free Protein Synthesis and Droplet Microfluidics” ACS Synthetic Biology 11(6): 2108-2120

160.

Liew F, Nogle R, Abdalla T, Rasor BJ, Canter C, Jensen RO, Wang L, Strutz J, Chirania P, De Tissera S, Mueller AP, Ruan Z, Gao A, Tran L, Engle NL, Bromley JC, Daniell J, Conrado R, Tschaplinski TJ, Giannone RJ, Hettich RL, Karmim AS, Simpson SD, Brown SD, Leang C, Jewett MC, Kopke M (2022) “Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale” Nature Biotechnology 40: 335-344

159.

Hunt AC, Case JB, Park Y-J, Cao L, Wu K, Walls AC, Liu Z, Bowen JE, Yeh H-W, Saini S, Helms L, Zhao YT, Hsiang T-Y, Starr TN, Goreshnik I, Kozodoy L, Carter L, Ravichandran R, Green LB, Matochko WL, Thomson CA, Vogeli B, Kruger A, Vanblargan LA, Chen RE, Ying B, Bailey AL, Kafai NM, Boyken SE, Ljubetic A, Edman N, Ueda G, Chow CM, Johnson M, Addetia A, Navarro MJ, Panpradist N, Galejr M, Freedman BS, Bloom JD, Ruohola-Baker H, Whelan SPJ, Stewart L, Diamond MS, Veesler D, Jewet MC, Baker D (2022) “Multivalent designed proteins neutralize SARS-CoV-2 variants of concern and confer protection against infection in mice” Science Translational Medicine 14(646)

158.

Zubi YS, Seki K, Li Y, Hunt AC, Liu B, Roux B, Jewett MC, Lewis JC (2022) “Metal-responsive regulation of enzyme catalysis using genetically encoded chemical switches” Nature Communications 13: 1864

157.

Rybnicky GA, Fackler NA, Karim AS, Kopke M, Jewett MC (2022) “Spacer2PAM: A computational framework to guide experimental determination of functional CRISPR-Cas system PAM sequences” Nucleic Acids Research 50(6): 3523-3534

156.

Tarasava K, Lee SH, Chen J, Kopke M, Jewett MC, Gonzalez R (2022) “Reverse β-oxidation pathways for efficient chemical production” Journal of Industrial Microbiology and Biotechnology 49(2): kuac003

155.

Rasor BJ, Vögeli B, Jewett MC, Karim AS (2022) “Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototyping” Cell-Free Gene Expression 2433: 199-215

154.

Rybnicky GA, Dixon RA, Kuhn M, Karim AS, Jewett MC (2022) “Development of a Freeze-Dried CRISPR-Cas12 Sensor for Detecting Wolbachia in the Secondary Science Classroom” ACS Synthetic Biology 11(2): 835-842

2021

153.

Hunt AC, Vogeli B, Kightlinger WK, Yoesep DJ, Kruger A, Jewett MC (2021) “A high-throughput, automated, cell-free expression and screening platform for antibody discovery” (Preprint)

152.

Kruger A, Watkins AM, Wellington-Oguri R, Romano J, Kofman C, DeFoe A, Kim Y, Anderson-Lee J, Fisker E, Townley J, Eterna participants, d’Aquino AE, Das R, Jewett MC (2021) “Community science designed ribosomes with beneficial phenotypes” (Preprint)

151.

Perez JG, Carlson ED, Weisser O, Kofman C, Seki K, Des Soye BJ, Karim A, Jewett MC (2021) “Improving genomically recoded Escherichia coli to produce proteins containing non-canonical amino acids” Biotechnology Journal 17(4): e2100330

150.

Rasor BJ, Yi X, Brown H, Alper HS, Jewett MC (2021) “An integrated in vivo/in vitro framework to enhance cell-free biosynthesis with metabolically rewired yeast extracts” Nature Communications 12: 5139

149.

Kofman C, Lee J, Jewett MC (2021) “Engineering molecular translation systems” Cell Systems 12(6): 593-607

148.

Meyer C, Nakamura Y, Rasor BJ, Karim AS, Jewett MC, Tan C (2021) “Analysis of the innovation trend in cell-free synthetic biology” Life 11(6): 551

147.

Hammerling MJ, Warfel KF, Jewett MC (2021) “Lyophilization of premixed COVID 19 diagnostic RT-qPCR reactions for rapid assembly and long-term stability” Biotechnology Journal16(7)

146.

Davis B, et al (2021) “Voices of Chemical Biology” Nature Chemical Biology 17: 1-4

145.

Liu Y, Davis RG, Thomas PM, Kelleher NL, Jewett MC (2021) “In vitro constructed ribosomes enable multi-site incorporation of non-canonical amino acids into proteins” Biochemistry 60(3): 161-169

144.

Hershewe JM, Warfel KF, Iyera SM, Peruzzi JA, Sullivan CJ, Rothe EW, DeLisa MP, Kamat NP, and Jewett MC (2021) “Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles” Nature Communications (12): 2363

143.

Fackler N, Heijstra BJ, Rasor BJ, Brown H, Martin J, Ni Z, Shebek KM, Rosin RR, Simpson SD, Tyo KE, Giannone RJ, Hettich RL, Tschaplinski T, Leang C, Brown SD, Jewett MC, Köpke M (2021) “Stepping on the Gas to a Circular Economy: Accelerating Development of Carbon-Negative Chemical Production from Gas Fermentation” Annual Review of Chemical and Biomolecular Engineering 12: Online

142.

Lee J, Schwarz K, Krüger A, Anslyn EV, Ellington A, Moore JS, Jewett MC (2021) “Ribosome-mediated incorporation of fluorescent amino acids into peptides in vitro” Chemical Communications57: 2661-2664 In Press

141.

Rasor BJ, Vögeli B, Landwehr GM, Bogart JW, Karim AS, Jewett MC (2021) “Toward sustainable, cell-free biomanufacturing” Current Opinion in Biotechnology 69: 136-144

140.

Stark JC, Jaroentomeechai T, Moeller TD, Hershewe JM, Warfel KF, Moricz BS, Martini AM, Dubner RS, Hsu KJ, Stevenson TC, Jones BD, DeLisa MP, Jewett MC (2021) “On-demand biomanufacturing of protective conjugate vaccines” Science Advances 7(6): eabe9444

2020

139.

Hammerling MJ, Yoesep DJ, Jewett MC (2020) “Single enzyme RT-PCR of full length ribosomal RNA” Synthetic Biology OUP5(1): ysaa028

138.

Gupta M, Wagner D, Jewett MC (2020) “Engineered proteins as multifunctional materials” MRS Bulletin 45(12): 999-1004

137.

Danielson N, McKay S, Bloom P, Dunn J, Jakel N, Bauer T, Hannon J, Jewett MC, Shanks B (2020) “Industrial Biotechnology—An Industry at an Inflection Point” Industrial Biotechnology 16(6): 321-332

136.

Hershewe JM, Wiseman WD, Kath JE, Buck CC, Gupta MK, Dennis PB, Naik RR, Jewett MC (2020) “Characterizing and controlling nanoscale self-assembly of suckerin-12” ACS Synthetic Biology 9(12): 3388-3399

135.

Hershewe JM, Kightlinger W, Jewett MC (2020) “Cell-free systems for accelerating glycoprotein expression and biomanufacturing” Journal of Industrial Microbiology and Biotechnology 47(11): 977-991

134.

Bogart JW, Cabezas MD, Vögeli B, Wong D, Karim AS, Jewett MC (2021) “Cell-free exploration of the natural product chemical space” ChemBioChem 22(1): 84-91

133.

Karim AS, Liew FE, Garg S, Vögeli B, Rasor BJ, Gonnot A, Pavan M, Juminaga A, Simpson SD, Köpke M, Jewett, MC (2020) “Modular cell-free expression plasmids to accelerate biological design in cells” Synthetic Biology OUP 5(1): ysaa019

132.

Zhu B, Gan R, Cabezas MD, Kojima T, Nicol R, Jewett MC, Nakano H (2020) “Increasing cell-free gene expression yields from linear templates in Escherichia coli and Vibrio natriegens extracts by using DNA-binding proteins” Bioengineering and Biotechnology 117(12): 3849-3857

131.

Liu WQ, Wu C, Jewett MC, Li J (2020) “Cell-free protein synthesis enables one-pot cascade biotransformation in an aqueous-organic biphasic system” Biotechnology and Bioengineering 117(12): 4001-4008

130.

Lee J, Schwarz KJ, Kim DS, Moore JS, Jewett MC (2020) “Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro” Nature Communications 11: 4304

129.

Thames AH, Wolniak KL, Stupp SI, Jewett MC (2020) “Principles learned from the international race to develop a safe and effective COVID-19 vaccine” ACS Central Science 6 (8): 1341–1347

128.

Jaroentomeechai T, Taw MN, Li M, Aquino A, Agashe N, Chung S, Jewett MC, DeLisa MP (2020) “Cell-free synthetic glycobiology: Designing and engineering glycomolecules outside of living cells” Frontiers in Chemistry 8: 645

127.

Grubbe WS, Rasor BJ, Krüger A, Jewett MC, Karim AS (2020) “Cell-free styrene biosynthesis at high titers” Metabolic Engineering 61: 89-95

126.

Dudley QM, Karim AS, Nash CJ, Jewett MC (2020) “In vitro prototyping of limonene biosynthesis using cell-free protein synthesis” Metabolic Engineering 61: 251-260

125.

Kightlinger W, Warfel K, DeLisa MP, Jewett MC (2020) “Synthetic Glycobiology: Parts, Systems, and Applications.” ACS Synthetic Biology 9(7): 1534–1562

124.

Krüger A, Mueller AP, Rybnicky GA, Engle NL, Yang ZK, Tschaplinski TJ, Simpson SD, Köpke M, Jewett MC (2020) “Development of a clostridia-based cell free system for prototyping genetic parts and metabolic pathways” Metabolic Engineering 62: 95-105

123.

Karim AS, Dudley QM, Juminga A, Yuan Y, Crowe SA, Heggestad JT, Garg S, Abdalla T, Grubbe W, Rasor B, Coar D, Torculas M, Krein M, Liew F, Quattlebaum A, Jensen RO, Stuart J, Simpson SD, Köpke M, Jewett MC (2020) “ In vitro prototyping and rapid optimization of biosynthetic enzymes for cellular design.” Nature Chemical Biology 16: 912–919

122.

Lee J, Torres R, Kim DS, Byrom M, Ellington AD, Jewett MC (2020) “Ribosomal incorporation of cyclic β-amino acids into peptides using in vitro translation” Chemical Communications 56: 5597-5600

121.

Whitehead TA, Banta S, Bentley WE, Betenbaugh MJ, Chane C, Clark DS, Hoeslig C, Jewett MC, Junkeri B, Koffas M, Kshirsagark R, Lewis A, Lid CT, Maranas C, Papoutsakis E, Prather KJ, Schaffer S, Segatori L, Wheeldon I (2020) “The importance and future of biochemical engineering” Biotechnology and Bioengineering 117(8): 2305-2318

120.

Aleksashin NA, Szal T, d’Aquino AE, Jewett MC, Mankin AM (2020) “A fully orthogonal system for protein synthesis in bacterial cells” Nature Communications 11: 1858

119.

Zhuang L, Huang S, Liu WQ, Karim AS, Jewett MC, Li, J. (2020) “Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin” Metabolic Engineering 60: 37-44

118.

Kennedy NW, Hershewe JM, Nichols TM, Roth EW, Wilke CD, Jewett MC, Tullman-Ercek D (2020) “Apparent size and morphology of bacterial microcompartments varies with technique” PLOS ONE 5(3): e0226395

117.

Jewett MC, Hammerling MJ (2020) “Ribosomes read new languages” Nature Chemical Biology 16: 486-488

116.

Chen Z, Kibler RD, Hunt A, Busch F, Pearl J, Jia M, VanAernum ZL, Wicky BIM, Dods G, Liao H, El-Samad H, Stamatoyannopoulos J, Wysocki VH, Jewett MC, Boyken SE, Baker D (2020) “De novo design of protein logic gates” Science 368: 78-84

115.

Thavarajah W, Verosloff MS, Jung JK, Alam KK, Miller JD, Jewett MC, Young SL, Lucks JB (2020) “A primer on emerging field-deployable synthetic biology tools for democratizing global water quality monitoring” NPJ Clean Water 3:18

114.

Hammerling MJ, Fritz BR, Yoesep DJ, Kim DS, Carlson ED, Jewett MC (2020) “In vitro ribosome synthesis and evolution through ribosome display” Nature Communications 11: 1108

113.

Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB (2020) “Design and optimization of a cell-free atrazine biosensor” ACS Synthetic Biology 9(3): 671-677

112.

Lin L, Kightlinger W, Hockenberry AJ, Jewett MC, Mrksich M (2020) “Sequential glycosylation of protein via substrate specific N-glycosyltransferases” ACS Central Science 6(2): 144-154

111.

d’Aquino AE, Azim T, Aleksashin NA, Hockenberry AJ, Jewett MC (2020) “Mutational characterization and mapping of the 70S ribosome active site” Nucleic Acids Research 48(5): 2777–2789

110.

Liu X, Silverman AD, Alam KK, Iverson E, Lucks JB, Jewett MC, Raman S (2020) “Design of a transcriptional biosensor for the portable, on-demand detection of cyanuric acid” ACS Synthetic Biology 9(1): 84-94

109.

Silverman AD, Karim AS, Jewett MC (2020) “Cell-free gene expression systems: An expanding repertoire of applications” Nature Reviews Genetics 21: 151-170

108.

Thavarajah W, Silverman AS, Verosloff MS, Kelley-Loughnane N, Jewett MC, Lucks JB (2020) “Point-of-use detection of environmental fluoride via an engineered cell-free riboswitch-based biosensor” ACS Synthetic Biology 9(1): 10-18

107.

Hammerling MJ, Krüger A, Jewett MC (2020) “Strategies for in vitro engineering of the translation machinery” Nucleic Acids Research 48(3): 1068-1083

2019

106.

Techner JM, Kightlinger W, Lin L, Hershewe J, Ramesh A, DeLisa MP, Jewett MC, Mrksich M (2019) “High-throughput synthesis and analysis of intact glycoproteins using SAMDI-MS” Analytical Chemistry 92(2): 1963-1971

105.

Kay JE, Jewett MC (2019) “A cell-free system for production of 2,3-butanediol is robust to growth-toxic compounds” Metabolic Engineering Communications 10: e00114

104.

Meyer A, Saaem I, Silverman AD, Varaljay V, Mikol R, Blum S, Onderko E, Bristol C, Liu S, Pratt K, Casini A, Eluere R, Moser F, Servinksy M, Lucks JB, Lux M, Glaven S, Crookes-Goodson W, Jewett MC, Gordon DB, Voigt CA (2019) “Organism engineering for the bioproduction of the triaminotrinitrobenzene (TATB) precursor phloroglucinol (PG)” ACS Synthetic Biology 8(12): 2746-2755

103.

Karim AS, Rasor BJ, and Jewett MC (2019) “Enhancing control of cell-free metabolism through pH modulation” Synthetic Biology 5(1): ysz027

102.

Kightlinger W, Duncker K, Ramesh A, Thames A, Natarajan A, Stark JC, Yang A, Lin L, Mrksich M, DeLisa MP, Jewett MC (2019) “A cell-free biosynthesis platform for modular construction of protein glycosylation pathways” Nature Communications 10: 5404

101.

Lee J, Schwieter KE, Watkins AM, Kim DS, Yu H, Schwarz KJ, Lim J, Coronado J, Byrom M, Anslyn EV, Ellington AD, Moore JS, Jewett MC (2019) “Expanding the limits of the second genetic code with ribozymes” Nature Communications 10: 5097

100.

Des Soye BJ, Gerbasi VR, Thomas PM, Kelleher NL, Jewett MC (2019) “A highly productive, one-pot cell-free protein synthesis platform based on genomically recoded Escherichia coli” Cell Chemical Biology 26: 1-12

99.

McNerney MP, Zhang Y, Steppe P, Silverman AD, Jewett MC, Styczynski MP (2019) “Point-of-care biomarker quantification enabled by sample-specific calibration” Science Advances 5 (9): eaax4473

98.

Carlson ED, d’Aquino AE, Kim DS, Fulk EM, Hoang K, Szal T, Mankin AS, Jewett MC (2019) “Engineered ribosomes with tethered subunits for expanding biological function” Nature Communications (10): 3920

97.

Yesselman JD, Eiler D, Carlson ED, Gotrik MR, d’Aquino AE, Ooms AN, Kladwang W, Carlson PD, Shi X, Costantino DA, Herschlag D, Lucks JB, Jewett MC, Kieft JS, Das R (2019) “Computational design of three-dimensional RNA structure and function” Nature Nanotechnology 14: 866-873

96.

Stark JC, Huang A, Hsu KJ, Dubner RS, Forbrook J, Marshalla S, Rodriguez F, Washington M, Rybnicky GA, Nguyen PQ, Hasselbacher B, Jabri R, Kamran R, Koralewski V, Wightkin W, Martinez T, Jewett MC (2019) “BioBits Health: Exploring engineering, biology, and human health with fluorescent readouts” ACS Synthetic Biology 8 (5):1001-1009

95.

O’Kane PT, Dudley QM, McMillan AK, Jewett MC, Mrksich M (2019) “High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA” Science Advances 5(6): eaaw9180

94.

Aleksashin NA, Leppik M, Hockenberry AJ, Klepacki D, Vázquez-Laslop N, Jewett MC, Remme J, Mankin AS (2019) “Assembly and functionality of the ribosome with tethered subunits” Nature Communications 10: 930

93.

Levine MZ, Gregorio NE, Jewett MC, Watts KR, Oza JP (2019) “Escherichia coli-based cell-free protein synthesis: protocols for a robust, flexible, and accessible platform technology” J. Vis. Exp. (144): e58882

92.

Dudley QM, Nash CJ, Jewett MC (2019) “Cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates” Synthetic Biology 4 (1): ysz003

2018

91.

Silverman AD, Kelley-Loughnane N, Lucks JB, Jewett MC (2018) “Deconstructing cell-free extract preparation for in vitro activation of transcriptional genetic circuitry” ACS Synthetic Biology 8 (2): 403-414

90.

Quillin SJ, Hockenberry AJ, Jewett MC, Seifert HS (2018) “Neisseria gonorrhoeae exposed to sublethal levels of hydrogen peroxide mounts a complex transcriptional response” mSystems 3 (5): e00156-18

89.

Caschera F, Karim AS, Gazzola G, d’Aquino AE, Packard NH, Jewett MC (2018) “High-throughput optimization cycle of a cell-free ribosome assembly and protein synthesis system” ACS Synthetic Biology 7 (12): 2841-2853

88.

Bundy BC, Hunt JP, Jewett MC, Swartz JR, Wood DW, Frey DD, Rao G (2018) “Cell-free biomanufacturing” Current Opinion in Chemical Engineering 22: 177-183

87.

Des Soye BJ, Davidson SR, Weinstock MT, Gibson DG, Jewett MC (2018) “Establishing a high-yielding cell-free protein synthesis platform derived from Vibrio natriegens” ACS Synthetic Biology 7(9): 2245-2255

86.

Hockenberry AJ, Jewett MC, Amaral LAN, Wilke CO (2018) “Within-gene Shine-Dalgarno sequences are not selected for function” Molecular Biology and Evolution: msy150

85.

Huang A, Nguyen PQ, Stark JC, Takahasi MK, Donghia N, Ferrante T, Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ (2018) “BioBits™ Explorer: A modular synthetic biology education kit” Science Advances 4: eaat5105

84.

Stark JC, Huang A, Nguyen PQ, Dubner RS, Hsu KJ, Ferrante T, Anderson M, Kanapskyte A, Mucha Q, Packett JS, Patel P, Patel R, Qaq D, Zondor T, Burke J, Martinez T, Miller-Berry A, Puppala A, Reichert K, Schmid M, Brand L, Hill LR, Chellaswamy JF, Faheem N, Fetherling S, Gong E, Gonzalzles EM, Granito T, Koritsaris J, Nguyen B, Ottman S, Palffy C, Patel A, Skweres S, Slaton A, Woods T, Donghia N, Pardee K, Collins JJ, Jewett MC (2018) “BioBits™ Bright: A fluorescent synthetic biology education kit” Science Advances 4(8): eaat5107

83.

Jaroentomeechai T, Stark JC, Natarajan A, Glasscock C, Yates L, Hsu KJ, Mrksich M, Jewett MC, DeLisa MP (2018) “Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery” Nature Communications 9: 2686

82.

JA Schoborg, MC Jewett (2018) “Cell‐free protein synthesis: An emerging technology for understanding, harnessing, and expanding the capabilities of biological systems” Synthetic Biology: Parts, Devices and Applications, 301-330

81.

Karim AS, Jewett MC (2018) “Cell-free synthetic biology for pathway prototyping” Methods in Enzymology 608: 31-57

80.

d’Aquino AE, Kim DS, Jewett MC (2018) “Engineered ribosomes for basic science and synthetic biology” Annual Review of Chemical and Biomolecular Engineering 9: 311-340

79.

Wang H, Li J, and Jewett MC (2018) “Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements” Synthetic Biology 3(1): ysy003

78.

Casini A, Chang F-Y, Eluere R, King A, Young E, Dudley QM, Karim A, Pratt K, Bristol C, Forget A, Ghodasara A, Warden-Rothman R, Gan R, Cristofaro A, Borujeni AE, Ryu M, Li J, Kwon YC, Wang H, Tatsis E, Rodriguez-Lopez C, O’Connor S, Medema MH, Fischbach MA, Jewett MC, Voigt C, Gordon DB (2018) “A pressure test to make 10 molecules in 90 days: External evaluation of methods to engineer biology” J. Am. Chem. Soc. 140 (12): 4302-4316

77.

Kightlinger W, Lin L, Rosztoczy M, DeLisa MP, Mrksich M, Jewett MC (2018) “Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases” Nature Chemical Biology 14: 627-635

76.

Martin RW, Des Soye BJ, Kwon YC, Kay J, Davis RG, Thomas PM, Marcum RD, Majewska NI, Chen CX, Weiss MG, Stoddart AE, Amiram M, Charna AK, Isaacs FJ, Kelleher NL, Hong SH, Jewett MC (2018) “Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids” Nature Communications 9: 1203

75.

Kakkar N, Perez JG, Liu W, Jewett MC, van der Donk W (2018) “Incorporation of non-proteinogenic amino acids in class I and II lantibiotics” ACS Chemical Biology 13: 951-957

74.

Aebersold R, Agar JN, Amster J, Baker M, Bertozzi CR, Boja ES, Costello CE, Cravatt BF, Fenselau C, Garcia B, Ge Y, Gunawardena J, Hendrickson RC, Hergenrother PJ, Huber C, Ivanov AR, Jensen O, Jewett MC, Kelleher NL, Kiessling LL, Krogan NJ, Larsen MR, Loo JA, Loo RR, Lundberg E, MacCoss M, Mallick P, Mootha VK, Mrksich M, Muir TW, Patrie SM, Pesavento JJ, Pitteri SJ, Rodriguez H, Saghatelian A, Sandoval W, Schlüter H, Sechi A, Sheeley DM, Slavoff SA, Smith LM, Snyder MP, Thomas PM, Uhlen M, van der Donk WA, van Eyk JE, Vidal M, Walt DR, White FM, Williams ER, Wohlschlager T, Wysocki VH, Yates NA, Young NL, Zhang B (2018) “How many human proteoforms are there?” Nature Chemical Biology 14(3): 206-214

73.

Liu SS, Hockenberry AJ, Lancichinetti A, Jewett MC, Amaral LAN (2018) “A novel framework for evaluating the performance of codon usage bias metrics” Journal of the Royal Society Interface 15(138): 20170667

72.

Liu CC, Jewett MC, Chin JW, Voigt CA (2018) “Towards an orthogonal central dogma” Nature Chemical Biology 14:103-106

71.

Schoborg JA, Hershewe J, Stark JC, Kightlinger W, Kath JE, Jaroentomeechai T, Natarajan A, DeLisa MP, Jewett MC (2018) “A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases ” Biotechnology and Bioengineering 115(3): 739-750

70.

Li J, Wang H, Jewett MC (2018) “Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields” Biochemical Engineering Journal 130: 29-33

69.

Hockenberry AJ, Stern AJ, Amaral LAN, Jewett MC (2018) “Diversity of translation initiation mechanisms across bacterial species is driven by environmental conditions and growth demands” Molecular Biology and Evolution 35 (3): 582-592

68.

Karim AS, Heggestad JT, Crowe SA, Jewett MC (2018) “Controlling cell-free metabolism through physiochemical perturbations” Metabolic Engineering 45: 86-94

2017

67.

Martin RW, Majewska NI, Chen CX, Albanetti TE, Jimenez RB, Schmelzer AE, Jewett MC, Roy V (2017) “Development of a CHO-based cell-free platform for synthesis of active monoclonal antibodies” ACS Synthetic Biology 6(7): 1370-1379

66.

Li J, Haas W, Jackson K, Kuru E, Jewett MC, Fan ZH, Gygi SP, and Church GM (2017) “Co-generating synthetic parts toward a self-replicating system” ACS Synthetic Biology 6(7): 1370-1379

65.

Jaroentomeechai T, Zheng X, Hershewe J, Stark JC, Jewett MC, and DeLisa MP (2017) “A pipeline for studying and engineering single-subunit oligosaccharyltransferases” Methods in Enzymology, Chemical Glycobiology 597: 55-81

64.

Liu Y, Kim DS, Jewett MC (2017) “Repurposing ribosomes for synthetic biology” Current Opinion in Chemical Biology 40: 87-94

63.

Li J, Wang H, Kwon YC, Jewett MC (2017) “Establishing a high yielding Streptomyces-based cell-free protein synthesis system” Biotechnology and Bioengineering 114(6): 1343-1353

62.

Hockenberry AJ, Pah AR, Jewett MC, Amaral LAN (2017) “Leveraging genome-wide datasets to quantify the functional role of the anti-Shine-Dalgarno sequence in regulating translation efficiency” Open Biology 7(1): 160239

61.

Gan R, Perez JG, Carlson ED, Ntai I, Isaacs FJ, Kelleher NL, Jewett MC (2017) “Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins” Biotechnology and Bioengineering 114(5): 1074-1086

2016

60.

Liu SS, Hockenberry AJ, Jewett MC, Amaral LAN (2016) “NullSeq: A tool for generating random coding sequences with desired amino acid and GC contents” PLoS Computational Biology 12(11): e1005184

59.

Yang C, Hockenberry AJ, Jewett MC, Amaral LAN (2016) “Depletion of Shine-Dalgarno sequences within bacterial coding regions is expression dependent” G3: Genes|Genomes|Genetics 6(11): 3467-3474

58.

Goering AW, Li J, McClure RA, Thomson RJ, Jewett MC, Kelleher NL (2016) “In vitro reconstruction of nonribosomal peptide biosynthesis directly from DNA using cell-free protein synthesis” ACS Synthetic Biology 6: 39-44

57.

Dudley QM, Anderson KC, Jewett MC (2016) “Cell-free mixing of Escherichia coli crude extracts to prototype and rationally engineer high-titer mevalonate synthesis” ACS Synthetic Biology 5(12): 1578-1588

56.

Karim AS, Jewett MC (2016) “A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery” Metabolic Engineering 36: 116-126

55.

Schoborg JA, Clark LG, Choudhury A, Hodgman CE, Jewett MC (2016) “Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis” Synthetic and Systems Biotechnology 1(1): 2-6

54.

Caschera F, Lee JW, Ho KKY, Liu AP, Jewett MC (2016) “Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes” Chemical Communications 52(31): 5467-5469

53.

Gan R, Jewett MC (2016) “Evolution of translation initiation sequences using in vitro yeast ribosome display” Biotechnology and Bioengineering 113:1777-1786

52.

Li J, Lawton TJ, Kostecki JS, Nisthal A, Fang J, Mayo SL, Rosenzweig AC, Jewett, MC (2016) “Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase” Biotechnology Journal 11: 212–218

2015

51.

Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW, Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Söll D, Rinehart J, Isaacs FJ (2015) “Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids” Nature Biotechnology 33: 1272-1279

50.

Sullivan CJ, Pendleton ED, Sasmor HH, Hicks WL, Farnum JB, Muto M, Amendt EM, Schoborg JA, Martin RW, Clark LG, Anderson MJ, Choudhury A, Fior R, Lo YH, Griffey RH, Chappell SA, Jewett MC, Mauro VP, Dresios J (2016) “A cell-free expression and purification process for rapid production of protein biologics” Biotechnology Journal 11: 238-248

49.

Kay JE, Jewett MC (2015) “Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2, 3-butanediol” Metabolic Engineering 32: 133-142

48.

Des Soye B, Patel J, Isaacs FJ, Jewett MC (2015) “Repurposing the translation apparatus for synthetic biology” Current Opinion in Chemical Biology 28: 83-90

47.

Oza JP, Aerni HR, Pirman NL, Barber KW, ter Haar CM, Rogulina S, Amrofell MB, Isaacs FJ, Rinehart J, Jewett MC (2015) “Robust production of recombinant phosphoproteins using cell-free protein synthesis” Nature Communications 6: 8168

46.

Orelle C, Carlson ED, Szal T, Florin T, Jewett MC, Mankin AS (2015) “Protein synthesis by ribosomes with tethered subunits” Nature 524: 119–124

45.

Anderson MJ, Stark JC, Hodgman CE, Jewett MC (2015) “Energizing yeast cell-free protein synthesis with glucose metabolism” FEBS Letters 589: 1723-1727

44.

Moatsou D, Li J, Ranji A, Pitto-Barry A, Ntai I, Jewett MC, O’Reilly RK (2015) “Reversible self-assembly of temperature-responsive protein-polymer conjugates” Bioconjugate Chemistry 26: 1890-1899

43.

Fritz BR, Jamil OK, and Jewett MC (2015) “Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction” Nucleic Acids Research 43: 4774-4784

42.

Heinzelman P, Schoborg JA, Jewett MC (2015) “pH responsive granulocyte colony stimulating factor variants with implications for treating Alzheimer’s disease and other central nervous system disorders” Protein Engineering Design and Selection 28: 481-489

41.

Hong SH, Kwon YC, Martin RW, Des Soye BJ, de Paz AM, Swonger KN, Ntai I, Kelleher NL, Jewett MC (2015) “Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking Release Factor 1″ ChemBioChem 16: 844-853 (inner cover)

40.

Kwon YC, Jewett MC (2015) “High-throughput preparation methods of crude extract for robust cell-free protein synthesis” Scientific Reports 5: 8663

39.

Ploskon E, Wagner S, Ellington AD, Jewett MC, O’Reilly R, Booth P (2015) “Controlled assembly of artificial protein-protein complexes via DNA duplex formation” Bioconjugate Chemistry 26: 427-434

38.

Dudley QM, Karim AS, Jewett MC (2015) “Cell-free metabolic engineering: Biomanufacturing beyond the cell” Biotechnology Journal 10: 69-82

2014

37.

Choudury A, Hodgman CE, Anderson M, Jewett MC (2014) “Evaluating fermentation effects on cell growth and crude extract metabolic activity for improved yeast cell-free protein synthesis” Biochemical Engineering Journal 91: 140-148

36.

Hodgman CE, Jewett MC (2014) “Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis” New Biotechnology 31: 499-505

35.

Liu Y, Fritz BR, Anderson MJ, Schoborg JA, Jewett MC (2014) “Characterizing and alleviating substrate limitations for improved in vitro ribosome construction” ACS Synthetic Biology 4: 454-462

34.

Fritz BR, Jewett MC (2014) “The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction” Nucleic Acids Research 42: 6774-6785

33.

Gan R, Jewett MC (2014) “A combined cell-free transcription-translation system from Saccharomyces cerevisiae for rapid and robust protein synthesis” Biotechnology Journal 9: 641-651

32.

Schoborg JA, Hodgman CE, Anderson M, Jewett MC (2014) “Characterizing substrate limitations for improved yeast cell-free protein synthesis” Biotechnology Journal 9: 631-640

31.

Hockenberry AJ, Sirer MA, Amaral LAN, Jewett MC (2014) “Quantifying position dependent codon usage bias” Molecular Biology and Evolution 31: 1880-1893

30.

Palmer MJ, Jewett MC (2014) “Enabling a next generation of synthetic biology community organization and leadership” ACS Synthetic Biology 3: 117-120 (Viewpoint)

29.

Hong SH, Ntai I, Haimovich AD, Kelleher NL, Isaacs FJ, Jewett MC (2014) “Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific non-standard amino acid incorporation” ACS Synthetic Biology 3: 398-409

28.

Hong SH, Kwon YC, Jewett MC (2014) “Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis” Frontiers in Chemistry 2: 34

2013

27.

Jewett MC, Workman CT, Nookaew I, Pizarro FP, Hellgren L, Nielsen J (2013) “Mapping condition  dependent regulation of lipid metabolism” G3: Genes|Genomes|Genetics 3: 1979-1995

26.

Hodgman CE, Jewett MC (2013) “Optimizing extract preparation and reaction conditions to increase yield and reduce costs in a yeast cell-free protein synthesis system” Biotechnology and Bioengineering 110: 2643-2654

25.

Jewett MC, Patolsky F (2013) “Nanobiotechnology: Synthetic biology meets materials science” Current Opinion in Biotechnology 24: 551-554 (Editorial Overview)

24.

Kang Y, Lu A, Ellington AD, Jewett MC, O’Reilly RK (2013) “Effect of complementary nucleobase interactions on the copolymer composition of RAFT copolymerizations” ACS Macro Letters 2: 581-586

23.

Jewett MC, Fritz BR, Timmerman LE, Church GM (2013) “In vitro integration of ribosomal RNA synthesis, ribosome assembly, and protein synthesis” Molecular Systems Biology 9: 678

2012

22.

Harris DC, Jewett MC (2012) “Cell-free biology: Exploiting the interface between synthetic biology and synthetic chemistry” Current Opinion in Biotechnology 23: 672-678

21.

Hockenberry AJ, Jewett MC (2012) “Synthetic in vitro circuits” Current Opinion in Chemical Biology 16: 261-269

20.

Hodgman CE, Jewett MC (2012) “Cell-free synthetic biology: Thinking outside the cell” Metabolic Engineering 14: 261-269

2011

19.

Carlson ED, Gan R, Hodgman CE, Jewett MC (2011) “Cell-free protein synthesis: Applications come of age” Biotechnology Advances 30: 1185-1194

18.

Wang HH, Huang P-Y, Xu G, Haas W, Marblestone A, Li J, Gygi S, Forster AC, Jewett MC, Church GM (2012) “In vivo tagging of enzyme ensembles with MAGE for in vitro single-pot multi-enzyme catalysis” ACS Synthetic Biology 1: 43-52

17.

Isaacs FJ, Carr PA, Wang HH, Lajoie MJ, Sterling B, Kraal L, Tolonen A, Gianoulis T, Goodman D, Reppas NB, Emig CJ, Bang D, Hwang SJ, Jewett MC, Jacobson JM, Church GM (2011) “Precise manipulation of chromosomes in vivo enables genome-wide codon replacement” Science 333: 348-353

2010

16.

Fritz BR, Timmerman LE, Daringer NM, Leonard JN, Jewett MC (2010) “Biology by design: From top to bottom and back” Journal of Biomedicine and Biotechnology 2010: 11, Article ID 232016

15.

Jewett MC, Forster AC (2010) “Update on designing and building minimal cells” Current Opinion in Biotechnology 21: 697-703

2004 – 2009

14.

Moxley JF, Jewett MC, Antoniewicz MR, Villas-Boas SG, Alper HS, Wheeler RT, Tong L, Hinnebusch AG, Ideker T, Nielsen J, Stephanopoulos GN (2009) “Linking high resolution metabolic flux phenotypes and transcriptional regulation in yeast modulated by the global regulator Gcn4p” PNAS 106: 6477-6482

13.

Jewett MC, Miller ML, Chen Y, Swartz JR (2009) “Continued protein synthesis at low [ATP] and [GTP] enables cell adaptation during starvation” Journal of Bacteriology 191: 1083-1091

12.

Usaite R, Jewett MC, Oliveira AP, Yates JR, Olsson L, Nielsen J (2009) “Reconstruction of the yeast Snf1 kinase regulatory network reveals its role as a global energy regulator” Molecular Systems Biology 5: 319

11.

Pizarro FJ, Jewett MC, Nielsen J, Agosin E (2008) “Physiological and transcriptional mapping of evolutionary differences between commercial and laboratory Saccharomyces cerevisiae strains” Applied Environmental Microbiology 74: 6358-6368

10.

Nookaew N, Jewett MC, Meechai A, Thammarongtham C, Laoteng K, Cheevadhanarak S, Nielsen J, Bhumiratana S (2008) “The genome-scale metabolic model iIN800 of Saccharomyces cerevisiae and its validation: A scaffold to query lipid metabolism” BMC Systems Biology 2: 71

9.

Fazio A, Jewett MC, Daran-Lapujade P, Mustacchi R, Usaite R, Pronk JT, Workman CT, Nielsen J (2008) “Transcription factor control of growth rate dependent genes in Saccharomyces cerevisiae: A three factor design” BMC Genomics 9: 341

8.

Nielsen J, Jewett MC (2008) “The impact of systems biology and metabolic engineering of Saccharomyces cerevisiae” FEMS Yeast Research 8: 122-31

7.

Jewett MC, Calhoun KA, Voloshin A, Wuu JJ, Swartz JR (2008) “An integrated cell-free metabolic platform for protein production and synthetic biology” Molecular Systems Biology 4: 220

6.

Jewett MC, Hofmann G, Nielsen J (2006) “Fungal metabolite analysis in genomics and phenomics” Current Opinion Biotechnology 17: 191-197

5.

Jewett MC, Oliveira AP, Patil KR, Nielsen J (2005) “The role of high-throughput transcriptome analysis in metabolic engineering” Biotechnology and Bioprocess Engineering 10: 385-399

4.

Jewett MC, Swartz JR (2004) “Substrate replenishment extends protein synthesis with an in vitro system designed to mimic the cytoplasm” Biotechnology and Bioengineering 87: 465-472

3.

Jewett MC, Swartz JR (2004) “Mimicking the Escherichia colicytoplasmic environment activates long-lived and efficient protein synthesis” Biotechnology and Bioengineering 86: 19-26

2.

JR Swartz, MC Jewett, KA Woodrow (2004) “Cell-free protein synthesis with prokaryotic combined transcription-translation” Recombinant Gene Expression 267: 169-182

1.

Jewett MC, ad Swartz JR (2004) “Rapid expression and purification of 100 nmol quantities of active protein using cell-free protein synthesis” Biotechnology Progress 20: 102-109

Book Chapters

9.

Karim AS, Dudley QM, and Jewett MC (2017) “Cell-free synthetic systems for metabolic engineering and biosynthetic pathway prototyping,” in Industrial Biotechnology, James C. Liao, editor. Wiley Biotechnology Series: 125-148

8.

Perez JG, Stark JC, Jewett MC (2016). “Cell-free synthetic biology: Engineering beyond the cell,” in Synthetic Biology, J. Craig Venter, Daniel Gibson, Hamilton Smith, Clyde Hutchison, editors. Cold Spring Harbor Laboratory Press: a023853

7.

Schoborg JA, Jewett MC (2015) “Cell-free protein synthesis: An emerging technology for understanding, harnessing, and expanding the capabilities of biological systems,” in Synthetic Biology, Sven Panke and Christina Smolke, editors. Wiley Biotechnology Series: 125-148

6.

Ranji A, Wu JC, Bundy BC, Jewett MC (2013) “Transforming synthetic biology with cell-free systems,” in Synthetic Biology, Huimin Zhao, editor. Waltham, MA, Elsevier: 277-301

5.

Oliveira AP, Jewett MC, Nielsen J (2007) “From gene expression to metabolic fluxes” in Introduction to Systems Biology, Sangdun Choi, editor. Totowa, NJ, Humana Press Inc: 37-68

4.

Nielsen J, Jewett MC (2007) “The role of metabolomics in systems biology” in Metabolomics: A powerful tool in systems biology, Jens Nielsen, Michael Jewett, editors. Topics in Current Genetics Series. Heidelberg, Germany, Springer: 1-10

3.

Jewett MC, Hansen ME, Nielsen J (2007) “Data acquisition, analysis, and mining: Integrative tools for discerning metabolic function in Saccharomyces cerevisiae” in Metabolomics: A powerful tool in systems biology, Jens Nielsen, Michael Jewett, editors. Topics in Current Genetics Series. Heidelberg, Germany, Springer: 159-187.

2.

Swartz JR, Jewett MC, Woodrow KA (2004) “Cell-free protein synthesis with prokaryotic combined transcription-translation” in Recombinant Protein Protocols, Paulina Balbas, Argelia Lorence, editors. Methods in Molecular Biology Series, Vol. 267. Totowa, NJ, Humana Press Inc: 169-182

1.

Jewett MC, Voloshin A, and Swartz JR (2002) “Prokaryotic systems for in vitro expression” in Gene Cloning and Expression Technologies”, Michael Weiner, Quinn Lu, editors. Westborough, MA, Eaton Publishing: 391-411

Patents

10.

Swartz JR, Jewett MC (2013) Improved methods of in vitro protein synthesis. Canadian Patent No. 2,496,437

9.

Calhoun KA, Jewett MC, and Swartz JR (2013) Improved methods of in vitro protein synthesis. Korean Patent No. 10-1232656

8.

Swartz JR, Jewett MC (2013) Methods of in vitro protein synthesis. U.S. Patent No. 8,357,529

7.

Bond R, Jewett MC, McAuliffe JC, and Ward DE (2010) Biocomposite comprising co-precipitate of enzyme, silicate and polyamine. U.S. Patent No. 7,642,077

6.

Swartz JR, Jewett MC (2010) A method for synthesis of polynucleotides and/or polypeptides. Indian Patent No. 239129

5.

Calhoun KA, Jewett MC, Swartz JR (2009) Improved methods of in vitro protein synthesis. Australian Patent No. 2004293798

4.

Swartz JR, Jewett MC (2009) Improved methods of in vitro protein synthesis. Australian Patent No. 2003259912

3.

Calhoun KA, Jewett MC, Swartz JR (2009) Improved methods of in vitro protein synthesis. New Zealand Patent No. 546961

2.

Swartz JR, Jewett MC (2009) Improved methods of in vitro protein synthesis. European Patent No. 1539948.